monoclonal mouse anti rat cd43  (Bio-Rad)

Journal: Cells

Article Title: Adipose Tissue Stem Cells (ASCs) and ASC-Derived Extracellular Vesicles Prevent the Development of Experimental Peritoneal Fibrosis.

doi: 10.3390/cells14060436

Figure Lengend Snippet: Figure 4. Representative photomicrographs of immunohistochemistry for macrophages (CD68 clone ED-1) and leucocytes (CD43) in PM samples of the studied groups (×400). (A) Macrophages in the PM in the control rats (n = 6) were rare. (B) In the PF samples (n = 8), significant macrophage infiltration was detected and prevented by treatment with ASCs (n = 7) (C) or EVs (n = 8) (D). Similar results were observed in relation to leukocyte infiltration in the PM. (E) Only a few leukocytes were present in normal PMs, whereas a marked number of leukocytes was identified in the thickened PMs (F). Treatment with ASCs (G) or EVs (H) abrogated the inflammatory cell infiltration. Quantitative analysis of (I) macrophage and (J) leucocyte infiltration in the peritoneal area of the animals in the experimental groups are presented as bar graphs. A one-way ANOVA statistical analysis was performed: * p < 0.01 vs. control, # p < 0.01 vs. PF.

Article Snippet: Samples were incubated overnight with the following primary antibodies: monoclonal mouse anti-rat CD68 clone ED-1 (Serotec, Raleigh, NC, USA) for macrophages, monoclonal mouse anti-rat CD43 (Serotec), considered to be a T-cell-associated marker, and monoclonal mouse anti-α smooth muscle actin (α-SMA) (Sigma, St Louis, MO, USA) for myofibroblasts.

Techniques: Immunohistochemistry, Control

Journal: eLife

Article Title: Senataxin and RNase H2 act redundantly to suppress genome instability during class switch recombination

doi: 10.7554/eLife.78917

Figure Lengend Snippet:

Article Snippet: Antibody , PE-Cy7-CD43 (rat anti-mouse monoclonal) , BD Pharmingen , Cat# 562866 , Flow cytometry (1:20).

Techniques: Recombinant, Dot Blot, Flow Cytometry, Selection

Journal: eLife

Article Title: Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria

doi: 10.7554/eLife.72116

Figure Lengend Snippet:

Article Snippet: Antibody , Biotin Rat Anti-Mouse CD43 (Rat monoclonal) , BD Biosciences , Cat# 20,737 , Flow cytometry (1/300).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay, Recombinant, Sequencing, Purification, Lysis, Transfection, Software

Journal: Immunity

Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development

doi: 10.1016/j.immuni.2020.06.006

Figure Lengend Snippet: (A) Mean GAC-reactive serum IgM antibody concentrations in C57BL/6 mice following immunization as 14-day-old pups (grey) or as 56-day-old adults (black), relative to levels in naïve adult mice (dotted line). (B) GAC-reactive antibody-secreting cell (ASC) numbers in spleen and BM of naïve (white, n=10) or d14 GAS-immunized mice (grey, n=9). Data are mean ± s.d. with individual data points overlaid, and representative ELISpot images embedded; ns (not significant), ***p<.001 by two-way ANOVA. (C) GAC+ B cell frequencies in spleen and peritoneal cavity (PerC) of C57BL/6 mice at neonatal and adult timepoints. (D) Phenotypic analysis of PerC GAC-binding B cells in naïve (top) and d14 GAS-immunized (bottom) mice; embedded frequencies are mean ± s.e.m. for n=7 naïve and n=8 d14 GAS-immunized mice, pooled from two replicate experiments. (E) Numbers of GAC+ B cells found in PerC, spleen and ILN of naïve (white, n=7) and d14 GAS-immunized (grey, n=8) adult mice. (F) Phenotypic distribution of PerC-localized GAC-binding B cells defined as CD5+CD43+CD23− B-1a B cells, CD5−CD43+CD23− B-1b B cells or CD5−CD43−CD23+ B-2 B cells. Data are pooled from two replicate experiments; ns (not significant), * p<.05, ***p<.001 by two-way ANOVA with Bonferroni’s multiple comparisons test. (G) CD11b expression by GAC+ B cells in naïve (n=4, white) and d14 GAS-immunized (n=4, grey) adult mice. (H) IdI-3a idiotype expression by GAC+ B cells in naïve (n=5) and d14 GAS-immunized (n=4) mice. Data are mean ± s.d. with individual data points from representative experiments completed three times; *p<.05, ** p<.01 by two-tailed t-test. See also Figure S1.

Article Snippet: Rat monoclonal anti-Mouse CD43 (Clone S7), FITC , BD Biosciences , Cat: 553270.

Techniques: Enzyme-linked Immunospot, Binding Assay, Expressing, Two Tailed Test

Journal: Immunity

Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development

doi: 10.1016/j.immuni.2020.06.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat monoclonal anti-Mouse CD43 (Clone S7), FITC , BD Biosciences , Cat: 553270.

Techniques: Recombinant, Sequencing, Software

Journal: Immunity

Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development

doi: 10.1016/j.immuni.2020.06.006

Figure Lengend Snippet: (A) Mean GAC-reactive serum IgM antibody concentrations in C57BL/6 mice following immunization as 14-day-old pups (grey) or as 56-day-old adults (black), relative to levels in naïve adult mice (dotted line). (B) GAC-reactive antibody-secreting cell (ASC) numbers in spleen and BM of naïve (white, n=10) or d14 GAS-immunized mice (grey, n=9). Data are mean ± s.d. with individual data points overlaid, and representative ELISpot images embedded; ns (not significant), ***p<.001 by two-way ANOVA. (C) GAC+ B cell frequencies in spleen and peritoneal cavity (PerC) of C57BL/6 mice at neonatal and adult timepoints. (D) Phenotypic analysis of PerC GAC-binding B cells in naïve (top) and d14 GAS-immunized (bottom) mice; embedded frequencies are mean ± s.e.m. for n=7 naïve and n=8 d14 GAS-immunized mice, pooled from two replicate experiments. (E) Numbers of GAC+ B cells found in PerC, spleen and ILN of naïve (white, n=7) and d14 GAS-immunized (grey, n=8) adult mice. (F) Phenotypic distribution of PerC-localized GAC-binding B cells defined as CD5+CD43+CD23− B-1a B cells, CD5−CD43+CD23− B-1b B cells or CD5−CD43−CD23+ B-2 B cells. Data are pooled from two replicate experiments; ns (not significant), * p<.05, ***p<.001 by two-way ANOVA with Bonferroni’s multiple comparisons test. (G) CD11b expression by GAC+ B cells in naïve (n=4, white) and d14 GAS-immunized (n=4, grey) adult mice. (H) IdI-3a idiotype expression by GAC+ B cells in naïve (n=5) and d14 GAS-immunized (n=4) mice. Data are mean ± s.d. with individual data points from representative experiments completed three times; *p<.05, ** p<.01 by two-tailed t-test. See also Figure S1.

Article Snippet: Rat monoclonal anti-Mouse CD43 (Clone S7), PE , BD Biosciences , Cat: 553271.

Techniques: Enzyme-linked Immunospot, Binding Assay, Expressing, Two Tailed Test

Journal: Immunity

Article Title: Neonatal exposure to commensal bacteria-derived antigens directs polysaccharide-specific B-1 B cell repertoire development

doi: 10.1016/j.immuni.2020.06.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat monoclonal anti-Mouse CD43 (Clone S7), PE , BD Biosciences , Cat: 553271.

Techniques: Recombinant, Sequencing, Software